Enhancement of Immune Effector Functions by Modulating IgG’s Intrinsic Affinity for Target Antigen

Enhancement of Immune Effector Functions by Modulating IgG’s Intrinsic Affinity for Target Antigen

Antibody-mediated immune effector functions play an essential role in the anti-tumor efficacy of many therapeutic mAbs. While much of the effort to improve effector potency has focused on augmenting the interaction between the antibody-Fc and activating Fc-receptors expressed on immune cells, the role of antibody binding interactions with the target antigen remains poorly understood. We show th...

Affinity for Target Antigen Cytotoxicity by IgG Intrinsic and Apparent Regulation of Antibody-Dependent Cellular

Regulation of antibody-dependent cellular cytotoxicity by IgG intrinsic and apparent affinity for target antigen.

Unconjugated mAbs have emerged as useful cancer therapeutics. Ab-dependent cellular cytotoxicity (ADCC) is believed to be a major antitumor mechanism of some anticancer Abs. However, the factors that regulate the magnitude of ADCC are incompletely understood. In this study, we described the relationship between Ab affinity and ADCC. A series of human IgG1 isotype Abs was created from the anti-H...

Production of antigen-specific human IgGs by in vitro immunization

BACKGROUND We previously developed in vitro immunization based on a fusion protein containing the transcriptional transactivator (Tat) of human immunodeficiency virus and a double domain, called ZZ, derived from protein A of Staphylococcus aureus. In this approach, naïve human peripheral blood mononuclear cells (PBMCs) trigger a specific IgM antibody (Ab) response in the presence of ZZTat. In t...

Proteolytically Inactivated IgGs Reconstitute the Effector Functions of Human Anti-IgG1 Hinge Autoantibodies